SubtiBank SubtiBank
Version comparison:

2018-05-30 18:01:122025-05-09 05:06:41

locus

BSU14990

BSU_14990

geneLength

447

450

function

control of [[protein|Rny|RNase Y]] activity

control of [[protein|Rny|RNase Y]] activity, see [SW|Targets of the Y complex]

outlinks

bsu

BSU14990

BSU_14990

Gene

Coordinates

1,568,420 → 1,568,869

1,568,420 1,568,869

Gene

Phenotypes of a mutant

strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

defective in [[protein|Rny|RNase Y]]-mediated maturation of polycistronic mRNAs [pubmed|29794222]

strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

defective in [[protein|Rny|RNase Y]]-mediated maturation of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

The protein

Catalyzed reaction/ biological activity

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex acts as specificity factor for [[protein|Rny|RNase Y]]-dependent processing of polycistronic mRNAs [pubmed|29794222]

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex stimulates [[protein|Spo0A]] phosphorylation in the [[category|phosphorelay]] [pubmed|27501195]

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex acts as specificity factor for [[protein|Rny|RNase Y]]-dependent processing of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex stimulates [[protein|Spo0A]] phosphorylation in the [[category|phosphorelay]] [pubmed|27501195]

Biological materials

Mutant

BKE14990 (Δ[[gene|ylbF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

BKK14990 (Δ[[gene|ylbF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

BKE14990 ([[gene|ylbF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

BKK14990 ([[gene|ylbF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

References

10712692, 15661000, 23490197, 15175311, 26434553, 28295778, 27501195, 29794222

The protein

[SW|Cofactors]

one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with [[protein|YmcA]] and [[protein|YaaT]])) [pubmed|31530674]

The protein

Structure

[PDB|6PRK] (the [[protein|YmcA]]-[[protein|YlbF]] complex) [pubmed|31530674]

Expression and Regulation

additional information

7,000/ 340 molecules per cell during growth in LB/ minimal medium [pubmed|31530674]

References

Reviews

32061882, 32156829, 32156813

References

Original publications

10712692, 15661000, 23490197, 15175311, 26434553, 28295778, 27501195, 29794222, 31530674